ReadyTector® reduces background and enables clear and sharp bands for most applications.
The results are suitable for publishing.
Lanes 1– 5 contain 22, 11, 5.5, 2.8, 1.4 ng Alpha-1-Antitrypsin spiked in cell lysates, respectively.
Protein detection: Mouse anti-A1AT Clone 1AT (Biotrend), 0.2 μg / mL on nitrocellulose.
The primary antibody for A1AT should only show one band. Additional weak signals show non-specific extra bands.